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Fibrinolysis mediated by tissue plasminogen activator. Disclosure of a kinetic transition

1985; Wiley; Volume: 149; Issue: 1 Linguagem: Inglês

10.1111/j.1432-1033.1985.tb08911.x

1432-1033

Bo Norrman, Per Wallén, Mats Rånby,

Blood properties and coagulation

The rate of 'Glu'-plasminogen activation by tissue plasminogen activator was repeatedly determined during a fibrinolytic process. The process was found to proceed via two distinct phases. The kinetics of each phase obeyed Michaelis-Menten equation: First phase; kcat about 0.17 s−1 and Km about 1 μM, second phase; kcat about 0.13 s−1 and Km about 0.06 μM. Practically identical results were obtained with one-chain as with two-chain tissue plasminogen activator. Transition from first to second phase occurred when the system had been exposed to a certain degree of plasmin digestion. Electrophoretic analysis demonstrated time correlation between the appearance of minimally degraded fibrin (X-fragments) and the transition. No such correlation was found between transition and conversion of 'Glu'-plasminogen to 'Lys'-plasminogen. The effect can result in an acceleration (up to 13-fold) of the fibrinolytic process once a slight degradation of the fibrin has taken place. In vivo, the effect described may constitute a mechanism that protects a fibrin clot from premature lysis.