Comparison of phenotypic methods for penicillinase detection in Staphylococcus aureus
2008; Elsevier BV; Volume: 14; Issue: 6 Linguagem: Inglês
10.1111/j.1469-0691.2008.01997.x
ISSN1469-0691
AutoresMartin Kaase, S. Lenga, Susanne Friedrich, Florian Szabados, Türkân Sakιnç, Britta Kleine, Sören Gatermann,
Tópico(s)Molecular Biology Techniques and Applications
ResumoPenicillinase testing is required for Staphylococcus aureus isolates with a penicillin MIC of </=0.12 mg/L. This study compared five phenotypic assays with a PCR for blaZ when testing 197 S. aureus isolates. The starch-iodine plate method and nitrocefin tests had low sensitivities of 42.9% and 35.7%, respectively. The cloverleaf assay and the penicillin zone-edge determination method had sensitivities of 67.8% and 71.4%, respectively, and these methods might be appropriate in many circumstances, but were not as sensitive as blaZ PCR.
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