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Mass Determination of Phosphoramidites

2006; Wiley; Volume: 26; Issue: 1 Linguagem: Inglês

10.1002/0471142700.nc1011s26

1934-9289

Shinya Harusawa, Mihoyo Fujitake, Takushi Kurihara, Zhengyun Zhao, David M.J. Lilley,

Mass Spectrometry Techniques and Applications

Abstract Nucleoside phosphoramidites are the most widely used building blocks in contemporary solid‐phase synthesis of oligonucleotides. The accurate molecular weight measurements of such molecules, which are acid‐labile compounds, may be easily determined by mass spectrometry using a matrix system, triethanolamine/NaCl, on a liquid secondary ion mass spectrometer (LSIMS) or fast‐atom bombardment (FAB) MS equipped with a double‐focusing mass spectrometer. The present method rapidly and easily measures the accurate molecular weights of various phosphoramidites as adduct ions [M+Na] + with an average mass error smaller than 0.4 ppm, allowing determination of the formulas of the phosphoramidites in place of elemental analysis. Further, it was found that intensities of molecular‐related ions could be enhanced to the highest degree by adjustment of the molar ratio of phosphoramidite and NaCl, fixing the amount of triethanolamine on LSIMS, making the present method a powerful tool for identification of phosphoramidites by mass spectrometry.