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In Vitro Establishment and Epiphyllous Plantlet Regeneration of Nymphaea `Daubeniana'

1990; American Society for Horticultural Science; Volume: 25; Issue: 12 Linguagem: Inglês

10.21273/hortsci.25.12.1664

2327-9834

Matthew A. Jenks, Michael J. Kane, F. J. Marousky, Dennis B. McConnell, Thomas Sheehan,

Botany, Ecology, and Taxonomy Studies

Water lilies (Nymphaea spp.) are the most popular water garden plants but are expensive to propagate.Traditional vegetative propagation techniques are inefficient, and losses to disease also contribute to the high costs.In vitro propagation of disease-free stock may be an effective method for combating disease losses, reducing propagation costs, and facilitating long-term germplasm storage.We found no published reports of establishment and multiplication of Nymphaea species in vitro.However, in vitro establishment of Nelumbo lutea, another member of the Nymphaeaceae, suggests that Nymphaea species may respond to in vitro propagation techniques (Kane et al., 1988).We found this to be the case for the tropical water lily Nymphaea 'Daubeniana' Hort.ex O. Thomas, which is described herein.Nymphaea 'Daubeniana', a sterile hybrid, reproduces asexually in situ via formation of epiphyllous plantlets from the adaxial surface of floating leaves above the point of petiole insertion (Masters, 1974).Leaves bearing immature plantlets 3 to 4 mm long were collected from plants maintained in tanks in a greenhouse.Epiphyllous plantlets were excised from donor leaves leaving a 3-mm-'diameter ring of leaf tissue surrounding the base of each plantlet.Explants were rinsed in flowing tap water for 30 min.The trichomes were removed and the explants were again rinsed for 30 min in tap water followed by immersion in 50% (v/v) ethanol for 90 sec and a 5-min rinse in sterile deionized water.Explants were agitated in 1.31% (v/v) aqueous sodium hypochlorite containing two drops of Tween-20 per 100 ml for 12 min followed by three successive 5-min rinses in sterile deionized water.Explants were individually transferred into 150 × 25mm culture tubes and cultured submersed in