Simultaneous Introduction of Multiple Mutations Using Overlap Extension PCR
1997; Future Science Ltd; Volume: 22; Issue: 1 Linguagem: Inglês
10.2144/97221bm03
ISSN1940-9818
Autores Tópico(s)CRISPR and Genetic Engineering
ResumoBioTechniquesVol. 22, No. 1 BenchmarksOpen AccessSimultaneous Introduction of Multiple Mutations Using Overlap Extension PCRLiming Ge & Peter RudolphLiming Ge*Address correspondence to Liming Ge, MorphoSys GmbH, Frankfurter Ring 193a, D-80807 München, Germany. Internet: E-mail Address: liming@morph.spacenet.de.MorphoSys GmbH, München, Germany & Peter RudolphMorphoSys GmbH, München, GermanyPublished Online:7 Aug 2018https://doi.org/10.2144/97221bm03AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "Simultaneous Introduction of Multiple Mutations Using Overlap Extension PCR." , 22(1), pp. 28–30FiguresReferencesRelatedDetailsCited ByCharacterization of a poly(butylene adipate-co-terephthalate) hydrolase from the mesophilic actinobacteria Rhodococcus fasciansPolymer Degradation and Stability, Vol. 184A two-step PCR assembly for construction of gene variants across large mutational distances2 April 2021 | Biology Methods and Protocols, Vol. 6, No. 1Detection of Weak Expression of SOLO DANCERS in the Male Germline Using CYCLIN-DEPENDENT KINASE A1 Coding Sequence1 January 2020 | Journal of Plant Growth Regulation, Vol. 39, No. 3Molecular insights into the role of the 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