Characterization of a novel metastatic prostate cancer cell line of LNCaP origin
2015; Wiley; Volume: 76; Issue: 2 Linguagem: Inglês
10.1002/pros.23115
ISSN1097-0045
AutoresMark Castanares, Ben T. Copeland, Wasim H. Chowdhury, Minzhi M. Liu, Ronald Rodríguez, Martin G. Pomper, Shawn E. Lupold, Catherine A. Foss,
Tópico(s)RNA Interference and Gene Delivery
ResumoThe ProstateVolume 76, Issue 2 p. 215-225 Original Article Characterization of a novel metastatic prostate cancer cell line of LNCaP origin Mark A. Castanares, Mark A. Castanares Department of Pharmacology and Molecular Sciences, Lilly Corporate Center, Indianapolis, IndianaMark A. Castanares and Ben T. Copeland contributed equally to this work.Search for more papers by this authorBen T. Copeland, Ben T. Copeland Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MarylandMark A. Castanares and Ben T. Copeland contributed equally to this work.Search for more papers by this authorWasim H. Chowdhury, Wasim H. Chowdhury The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorMinzhi M. Liu, Minzhi M. Liu The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorRonald Rodriguez, Ronald Rodriguez The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorMartin G. Pomper, Martin G. Pomper Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MarylandSearch for more papers by this authorShawn E. Lupold, Shawn E. Lupold The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorCatherine A. Foss, Corresponding Author Catherine A. Foss Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland Correspondence to: Catherine A. Foss, Russell H Morgan Department of Radiology and Radiological Sciences, CRB2 493, Johns Hopkins University School of Medicine, Baltimore, MD, 21228. E-mail: cfoss1@jhmi.eduSearch for more papers by this author Mark A. Castanares, Mark A. Castanares Department of Pharmacology and Molecular Sciences, Lilly Corporate Center, Indianapolis, IndianaMark A. Castanares and Ben T. Copeland contributed equally to this work.Search for more papers by this authorBen T. Copeland, Ben T. Copeland Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MarylandMark A. Castanares and Ben T. Copeland contributed equally to this work.Search for more papers by this authorWasim H. Chowdhury, Wasim H. Chowdhury The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorMinzhi M. Liu, Minzhi M. Liu The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorRonald Rodriguez, Ronald Rodriguez The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorMartin G. Pomper, Martin G. Pomper Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MarylandSearch for more papers by this authorShawn E. Lupold, Shawn E. Lupold The James Buchanan Brady Urologic Institute and Department of Urology, Johns Hopkins School of Medicine, Baltimore, MarylandSearch for more papers by this authorCatherine A. Foss, Corresponding Author Catherine A. Foss Russell H Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland Correspondence to: Catherine A. Foss, Russell H Morgan Department of Radiology and Radiological Sciences, CRB2 493, Johns Hopkins University School of Medicine, Baltimore, MD, 21228. E-mail: cfoss1@jhmi.eduSearch for more papers by this author First published: 26 October 2015 https://doi.org/10.1002/pros.23115Citations: 21 Current address of Mark A. Castanares: Lilly Corporate Center., Indianapolis, Indiana. Current address of Wasim H. Chowdhury and Ronald Rodriguez: University of Texas, San Antonio. Disclosures: There are no affiliations or conflicts to disclose. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract BACKGROUND The LNCaP cell line was originally isolated from the lymph node of a patient with metastatic prostate cancer. Many cell lines have been derived from LNCaP by selective pressures to study different aspects of prostate cancer progression. When injected subcutaneously into male athymic nude mice, LNCaP and its derivatives rarely metastasize. METHODS Here, we describe the characteristics of a new LNCaP derivative, JHU-LNCaP-SM, which was generated by long term passage in normal cell culture conditions. RESULTS Short tandem repeat (STR) analysis and genomic sequencing verified JHU-LNCaP-SM derivation from parental LNCaP cells. JHU-LNCaP-SM cells express the same mutated androgen receptor (AR) but unlike LNCaP, are no longer androgen dependent for growth. The cells demonstrate an attenuated androgen responsiveness in transcriptional assays and retain androgen sensitive expression of PSA, AR, and PSMA. Unlike parental LNCaP, JHU-LNCaP-SM cells quickly form subcutaneous tumors in male athymic nude mice, reliably metastasize to the lymph nodes and display a striking intra-tumoral and spreading hemorrhagic phenotype as tumor xenografts. CONCLUSIONS The JHU-LNCaP-SM cell line is a new isolate of LNCaP, which facilitates practical, preclinical studies of spontaneous metastasis of prostate cancer through lymphatic tissues. Prostate 76:215–225, 2016. © 2015 Wiley Periodicals, Inc. Citing Literature Supporting Information Additional supporting information may be found in the online version of this article at the publisher's web-site. Filename Description pros23115-sup-0001-SupFig-S1.tif5.2 MB Figure S1. In vitro immunostaining for PSMA and CD31 expression in LNCaP xenografts. pros23115-sup-0002-SupFig-S2.tif4 MB Figure S2. In vitro immunostaining for PSMA and CD31 expression in JHU-LNCaP-SM xenografts. pros23115-sup-0003-SupFig-S3.tif3.6 MB Figure S3. In vitro immunostaining for PSMA and CD31 expression of C4-2 xenografts. pros23115-sup-0004-SupFig-S4.tif5.7 MB Figure S4. In vitro immunostaining for PSMA and CD31 expression of JHU-LNCaP-SM lymph node metastasis. pros23115-sup-0005-SupFig-S5.tif875 KB Figure S5. Whole Western blot showing full-length androgen receptor expression in parental LNCaP, C4-2, and JHU-LNCaP-SM cultured cells. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume76, Issue2February 1, 2016Pages 215-225 RelatedInformation
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