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Application of immunohistochemistry to cytology.

2008; American Medical Association; Volume: 132; Issue: 3 Linguagem: Inglês

10.1043/1543-2165(2008)132[373

1543-2165

Larry J. Fowler, Whitney A. Lachar,

HER2/EGFR in Cancer Research

Abstract Context.—The uses of monoclonal antibodies via immunochemistry have been reported frequently within the literature using various methodologies with applications to cytology specimens. The direct application of immunochemistry to cytology may have a variety of pitfalls that the general pathologist familiar with its application to histology may be unaware of when applying it prospectively to patient specimens. Objective.—To review common pitfalls when applying immunochemistry to cytology specimens and to suggest approaches to the more common differential dilemmas that apply to a variety of cytology specimens that could be seen in a general pathology practice. Data Sources.—The authors' own experiences of applying immunochemistry to cytopathology specimens within an academic setting along with supportive data from the literature. Conclusions.—Immunochemistry can be used to increase the predictability of a cytology diagnosis if care is taken with the cytology sample preparation methodology and there ...