Follicular fluid contents as predictors of success of in-vitro fertilization-embryo transfer
1987; Oxford University Press; Volume: 2; Issue: 6 Linguagem: Inglês
10.1093/oxfordjournals.humrep.a136579
ISSN1460-2350
AutoresEran Rom, Nancy C. Reich, Neri Laufer, A. Lewin, R. Rabinowitz, Bella Pevsner, M Lancet, Joseph G. Shenker, Ruth Miskin, B C Adelmann-Grill, A. Tsafriri,
Tópico(s)Assisted Reproductive Technology and Twin Pregnancy
ResumoIn order to establish criteria for selection of the best ova in in-vitro fertilization-embryo transfer (IVF-ET) programmes we have examined the follicular fluid (FF) levels of plasminogen activator (PA), collagenolytic activity, progesterone (P) and α2 macroglobulin (α2M) and related them to the success of pregnancy. PA activity was similar in FF of pregnant and nonpregnant cycles, 13.8 ± 3.9 mU/ml versus 14.6 + 2.9 (mean ± SEM) respectively. By contrast, FF from pregnant cycles exhibited lower collagenolytic activity (49.6 ± 3.9% versus 67.9 ± 3.0; P < 0.001). Likewise, in a semi-quantitative assay of α2M, only 18.4% of the aspirates from pregnant cycles showed a precipitation line, whereas 76.8% of those from non-pregnant cycles were positive. Levels of P in aspirates from pregnant cycles were in the intermediate range, as compared with those from non-pregnant cycles (0.06–5.5 μg/ml versus 0.02–12.0 μg/ml). All these assays can be completed before ET and performed in IVF-ET programmes. In conclusion, it seems that a combination of follicular α2M levels and collagenolytic activity, and to a lesser extent addition of P assay, may serve as good criteria for selecting the best embryos for establishment of pregnancy.
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