Portal de Periódicos da CAPES

Capacidad infectiva de promastigotes en fase estacionaria de Leishmania (Viannia) braziliensis y Leishmania (Viannia) peruviana, en línea celular DH82

2015; National Institute of Health of Peru; Volume: 32; Issue: 1 Linguagem: Inglês

10.17843/rpmesp.2015.321.1572

1726-4642

Karen Daphne Calvay-Sánchez, Nyshon Rojas-Palomino, Aidé Sandoval-Juárez, Alberto Cisneros-Tarmeño, Cristian Obregón-Cahuaya, Gloria Minaya-Gómez,

Insect Pest Control Strategies

Objectives. To determine the infectivity of promastigotes of Leishmania (V.) peruviana and Leishmania (V.) braziliensis in monocyte-macrophage cell line DH82 of Canis familiaris. Materials and methods. Was conducted a experimental study during the months of january to december 2013. Were used strains of Leishmania were used (V.) braziliensis MHOM / PE / 84 / LC53 and Leishmania (V.) peruviana MHOM / PE / 84 / LC26. The cell line was infected with stationary phase promastigotes and infectivity was determined as the product of percent infected macrophages average amastigotes per macrophage observed in epifluorescence microscope. Results. 13% of metacyclic forms to Leishmania (V.) braziliensis corresponded to 17.5 days post inoculation and Leishmania (V.) peruviana a percentage of 9.5% on the day 14.5. No significant difference was found between infectivity of stationary phase promastigotes of both species. Conclusions. It is recommended assess the infectivity of metacyclic promastigotes peruviana strains of Leishmania (V.) and Leishmania (V.) braziliensis cell lines in order to determine the most appropriate model in vitro infection, allowing leishmanicidas make the drug more effective susceptibility studies for disease control.