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Crecimiento y producción de lacasa extracelular en cultivos líquidos de Minimidochium parvum cepa LPSC # 548

2007; Sociedad Argentina de Botánica; Volume: 42; Linguagem: Inglês

1851-2372

Mario Carlos Nazareno Saparrat, Angélica M. Arambarri, Pedro Alberto Balatti,

Analytical chemistry methods development

Minimidochium parvum LPSC # 548, a fungus isolated from litter floating on waters of Rio Santiago (Provincia de Buenos Aires, Argentina) polluted with industrial effluents and crude-oil, was grown as a shaking culture on a C-limited medium to evaluate its ability to produce extracellular laccase. The effect of anthracene, CuSO4 · 5H2O, ethanol, guaiacol, humic acids, Kraft lignin, MnSO4 · H2O, Tween 20 and veratryl alcohol on its growth and extracellular laccase activity levels was also analyzed. The cultures grown on basal medium produced maximum biomass (over 420 mg/100 ml) and maximum extracellular laccase activity (351.7 ±53.3 pkat/ml) after 5 days of incubation. Among the different factors tested, only the humic acids at 0.1 % (w/v) were found to stimulate the growth of M. parvum. However, Tween 20 (0.1 %, v/v) was the only one that produced an increase of laccase activity levels up to 2.5-fold compared to the control.