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EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL CAPACITY OF SYZYGIUM CUMINI L. LEAVES EXTRACTED SEQUENTIALLY IN DIFFERENT SOLVENTS

2011; Wiley; Volume: 37; Issue: 2 Linguagem: Inglês

10.1111/j.1745-4514.2011.00614.x

1745-4514

Mital J. Kaneria, Sumitra Chanda,

Ethnobotanical and Medicinal Plants Studies

Journal of Food BiochemistryVolume 37, Issue 2 p. 168-176 Full Article EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL CAPACITY OF SYZYGIUM CUMINI L. LEAVES EXTRACTED SEQUENTIALLY IN DIFFERENT SOLVENTS MITAL KANERIA, MITAL KANERIA Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot-360 005 Gujarat, IndiaSearch for more papers by this authorSUMITRA CHANDA, Corresponding Author SUMITRA CHANDA Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot-360 005 Gujarat, India TEL: +919426247892; EMAIL: [email protected]Search for more papers by this author MITAL KANERIA, MITAL KANERIA Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot-360 005 Gujarat, IndiaSearch for more papers by this authorSUMITRA CHANDA, Corresponding Author SUMITRA CHANDA Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University, Rajkot-360 005 Gujarat, India TEL: +919426247892; EMAIL: [email protected]Search for more papers by this author First published: 30 December 2011 https://doi.org/10.1111/j.1745-4514.2011.00614.xCitations: 28Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract ABSTRACT Syzygium cumini is commonly used in India as a traditional medicine. In this study, the antioxidant and antimicrobial potentials of different solvent extracts of S. cumini leaves were investigated. Extraction was done sequentially in Soxhlet apparatus, using various solvents (petroleum ether, toluene, ethyl acetate, acetone and water). Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, hydroxyl radical scavenging assay, superoxide anion radical scavenging assay and reducing capacity assessment. Total phenol and flavonoid content was also measured. The antimicrobial activity was done by agar well diffusion method against some of the tested foodborne, pathogenic and skin disease causing microorganisms. The acetone extract had more total phenol content and more antioxidant activities, conforming to the hypothesis that phenol content and antioxidant activity has a direct correlation. The acetone extract had notable antimicrobial effect on microorganisms. The results obtained appeared to confirm the antioxidant and antimicrobial potentials of the S. cumini. PRACTICAL APPLICATIONS Food safety is a fundamental concern of both consumers and the food industry, especially as the number of reported cases of food-associated infections continues to increase. It has been estimated that as many as 30% of people in industrialized countries suffer from foodborne diseases each year. Microorganisms play a major role in contamination of stored foods deteriorating them quantitatively as well as qualitatively. Fungi are significant destroyer of foodstuffs during storage, rendering them unfit for human consumption by retarding their nutritive value and sometimes by producing mycotoxins. In the present study, antioxidant and antimicrobial activities of different solvent extracts of Syzygium cumini leaves was evaluated. Acetone extract had good antioxidant activity comparable with standard and remarkable antimicrobial activity. The results indicate that S. cumini may become important natural source of compounds with health protective potential and antimicrobial agent, which can be used in pharmaceutical, nutraceutical and food preparation. REFERENCES AI-BARI, M.A., SAYEED, M.A., RAHMAN, M.S. and MOSSADIK, M.A. 2006. Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis a noval specis collected in Bangladesh. Respir. J. Med. Sci. 1, 77– 81. AJILA, C.M., NAIDU, K.A., BHAT, S.G. and PRASAD RAO, U.J.S. 2007. Bioactive compounds and antioxidant potential of mango peel extract. Food Chem. 105, 982– 988. ALESIANI, D., CANINI, A., D'ABROSCA, B., DELLAGRECA, M., FIORENTINO, A., MASTELLONE, C., MONACO, P. and SEVERINA, P. 2010. Antioxidant and antiproliferative activities of phytochemicals from Quince (Cydonia vulgaris) peels. Food Chem. 118, 199– 207. ATHUKORALA, Y., KIM, K.N. and JEON, Y.J. 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga Ecklonia cava. Food Chem. Toxicol. 44, 1065– 1074. BARAVALIA, Y., KANERIA, M., VAGHASIYA, Y., PAREKH, J. and CHANDA, S. 2009. Antioxidant and antibacterial activity of Diospyros ebenum Roxb. leaf extracts. Turk. J. Biol. 33, 159– 164. BARTOLOME, A., MANDAPA, K., DAVID, K.J., SEVILLA, I.I.I. and VILLANUEVA, J. 2006. SOS-red fluorescent protein (RFP) bioassay system for monitoring of antigenotoxic activity in plant extracts. Biosens. Bioelectron. 21, 2114– 2120. BRAGA, F.G., BOUZADA, M.L.M., FABRI, R.L., MATOS, M.O., MOREIRA, F.O., SCIO, E. and COIMBRA, E.S. 2007. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil. J. Ethnopharmacol. 111, 396– 402. BRITO, F.A., LIMA, L.A., RAMOS, M.F.S., NAKAMURA, M.J., CAVALHER-MACHADO, S.C., SIANI, A.C., HENRIQUES, M.G.M.O. and SAMPAIO, A.L.F. 2007. Pharmacological study of anti-allergic activity of Syzygium cumini (L.) Skeels. Braz. J. Med. Biol. Res. 40, 105– 115. CHANDA, S. and BARAVALIA, Y. 2010. Screening of some plant extracts against some skin diseases caused by oxidative stress and microorganisms. Afr. J. Biotechnol. 9, 3210– 3217. CHANDA, S. and DAVE, R. 2009. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Afr. J. Microbiol. Res. 3, 981– 996. CHANDA, S.V. and NAGANI, K.V. 2010. Antioxidant capacity of Manilkara zapota L. leaves extracts evaluated by four in vitro methods. Nature Sci. 8, 260– 266. CHANG, C., YANG, M., WEN, H. and CHERN, J. 2002. Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. J. Food Drug Anal. 10, 178– 182. DURAIPANDIYAN, V., AYYANAR, M. and IGNACIMUTHU, S. 2006. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement. Altern. Med. 6, 35– 41. EBRAHIMABADI, A.H., EBRAHIMABADI, E.H., DJAFARI-BIDGOLI, Z., KASHI, F.J., MAZOOCHI, A. and BATOOLI, H. 2010. Composition and antioxidant and antimicrobial activity of the essential oil and extracts of Stachys inflata Benth from Iran. Food Chem. 119, 452– 458. FRITSCHE, T.R., SADER, H.S., STILWELL, M.G., DOWZICKY, M.J. and JONES, R.N. 2005. Antimicrobial activity of tigecycline tested against organisms causing community-acquired respiratory tract infection and nosocomial pneumonia. Diagn. Microbiol. Infect. Dis. 52, 187– 193. GHOLIVAND, M.B., MEHDI, R.N., HOSSEIN, B. and ABDOLRASOUL, H.E. 2010. Chemical composition and antioxidant activities of the essential oil and methanol extracts of Psammogeton canescens. Food Chem. Toxicol. 48, 24– 28. JAGETIA, G.C. and BALIGA, M.S. 2003. Evaluation of the radioprotective effect of the leaf extract of Syzygium cumini (Jamun) in mice exposed to a lethal dose of gamma-irradiation. Nahrung 47, 181– 185. KANERIA, M., BARAVALIA, Y., VAGHASIYA, Y. and CHANDA, S. 2009. Determination of antibacterial and antioxidant potential of some medicinal plants from Saurashtra region India. Indian J. Pharm. Sci. 71, 406– 412. KRISHNARAJU, A.V., RAO, T.V.N., SUNDARARAJU, D., VANISREE, M., TSAY, H.S. and SUBBARAJU, G.V. 2006. Biological screening of medicinal plants collected from Eastern Ghats of India using Artemia salina (brine shrimp test). Int. J. Appl. Sci. Eng. 4, 115– 125. KUMAR, A., PADMANABHAN, N. and KRISHNAN, M.R.V. 2007. Central nervous system activity of Syzygium cumini seed. Pak. J. Nutr. 6, 698– 700. KUMAR, A., ILAVARASAN, R., JAYACHANDRAN, T., DEECARAMAN, M., ARAVINDAN, P., PADMANABHAN, N. and KRISHAN, M.R.V. 2008a. Anti-diabetic activity of Syzygium cumini and its isolated compound against streptozotocin-induced diabetic rats. J. Med. Plant Res. 2, 246– 249. KUMAR, A., ILAVARASAN, R., JAYACHANDRAN, T., DEECARAMAN, M., KUMAR, R.M., ARAVINDAN, P., PADMANABHAN, N. and KRISHAN, M.R.V. 2008b. Anti-inflammatory activity of Syzygium cumini seed. Afr. J. Biotechnol. 7, 941– 943. KUNCHANDY, E. and RAO, M.N.A. 1990. Oxygen radical scavenging activity of curcumin. Int. J. Pharm. 58, 237– 240. LIMA, L.A., SIANI, A.C., BRITO, F.A., SAMPAIO, A.L.F., HENRIQUES, M.G.M.O. and RIEHL, C.A.S. 2007. Correlation of anti-inflammatory activity with phenolic content in the leaves of Syzygium cumini (L.) Skeels (Myrtaceae). Quim. Nova. 30, 860– 864. LIN, J., OPOKU, A.R., GEHEEB-KELLER, M., HUTCHINGS, A.D., TERBLANCHE, S.E., JAGER, A.K. and VAN STADEN, J. 1999. Preliminary screening of some traditional Zulu medicinal plants for anti-inflammatory and antimicrobial activities. J. Ethnopharmacol. 68, 267– 274. MC CUNE, L.M. and JOHNS, T. 2002. Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. J. Ethnopharmacol. 82, 197– 205. MC DONALD, S., PRENZLER, P.D., ANTOLOVICH, M. and ROBARDS, K. 2001. Phenolic content and antioxidant activity of olive extracts. Food Chem. 73, 73– 84. NAMIKI, M. 1990. Antioxidants/antimutagens in foods. CRC Crit. Rev. Food Sci. Nutr. 29, 273– 300. NATHWANI, D. 2005. Tigecycline: Clinical evidence and formulary positioning. Int. J. Antimicrob. Agents 25, 185– 192. OLIVEIRA, I., SOUSA, A., VALENTÃO, P., ANDRADE, P.B., FERREIRA, I.C.F., FERRERES, F., BENTO, A., SEABRA, R., ESTEVINHO, L. and PEREIRA, J.A. 2007. Hazel (Corylus avellana L.) leaves as source of antimicrobial and antioxidative compounds. Food Chem. 105, 1018– 1025. OLIVEIRA, I., SOUSA, A., MORAIS, J.S., FERREIRA, I.C.F., BENTO, A., ESTEVINHO, L. and PEREIRA, J.A. 2008. Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food Chem. Toxicol. 46, 1801– 1807. PAREKH, J. and CHANDA, S. 2007. In vitro antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower (Lythraceae). Braz. J. Microbiol. 38, 204– 207. PAREKH, J., JADEJA, D. and CHANDA, S. 2005. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk J. Biol. 29, 203– 210. PARK, E.J. and JHON, D.Y. 2010. The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts. LWT – Food Sci. Technol. 43, 655– 659. PATEL, S.S., SHAH, R.S. and GOYAL, R.K. 2009. Antihyperglycemic, antihyperlipidemic and antioxidant effects of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rats. Indian J. Exp. Biol. 47, 564– 570. PEREZ, C., PAUL, M. and BAZERQUE, P. 1990. An antibiotic assay by the agar well diffusion method. Acta. Bio. Med. Exp. 15, 113– 115. RAJASEKARAN, M., BAPNA, J.S., LAKSHMANAN, S., NAIR, A.G.R., VELIATH, A.J. and PANCHANADAM, M. 1988. Antifertility effect in male rats of oleanolic acid, a triterpene from Eugenia jambolana flowers. J. Ethnopharmacol. 24, 115– 121. RAMIREZ, R.O. and RAO, C.C. Jr. 2003. The Gastroprotective effect of tannins extracted from duhat (Syzygium cumini Skeels) bark on HCl/ethanol induced gastric mucosal injury in Sprague-Dawley rats. Clin. Hemorheol. Microcirc. 29, 253– 261. ROBAK, J. and GRYGLEWSKI, R.J. 1988. Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol. 37, 837– 841. ROJAS, J.J., OCHOA, V.J., OCAMPO, S.A. and MONOZ, J.F. 2006. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in treatment of non-nosocomial infections. BMC Complement. Altern. Med. 6, 2– 7. RUAN, Z.P., ZHANG, L.L. and LIN, Y.M. 2008. Evaluation of the antioxidant activity of Syzygium cumini leaves. Molecules 13, 2545– 2556. SHAFI, P.M., ROSAMMA, M.K., JAMIL, K. and REDDY, P.S. 2002. Antibacterial activity of Syzygium cumini and Syzygium travancoricum leaf essential oils. Fitoterapia 73, 414– 416. SILVA, F.A.M., BORGES, F., GUIMARAES, C., LIMA, J.L.F.C., MATOS, C. and REIS, C. 2000. Phenolic acids and derivatives: Studies on the relationship among structure, radical scavenging activity and physicochemical parameters. J. Agric. Food Chem. 48, 2122– 2126. VAGHASIYA, Y. and CHANDA, S. 2007. Screening of methanol and acetone extracts of fourteen Indian medicinal plants for antimicrobial activity. Turk J. Biol. 31, 243– 248. VAGHASIYA, Y. and CHANDA, S. 2010. Antimicrobial and free radical scavenging activity of different solvent extracts of Mangifera indica L. seeds. Res. J. Microbial. 5, 1207– 1212. WIART, C., HANNAH, A., YASSIM, M., HAMIMAH, H. and SULAIMAN, M. 2004. Antimicrobial activity of Acalyoha siamensis Oliv. Ex Gage. J. Ethnopharmacol. 95, 285– 286. WISEMAN, H. and HALLIWELL, B. 1996. Damage to DNA by reactive oxygen and nitrogen species: Role of inflammatory disease and progression to cancer. Biochem. J. 313, 17– 29. YAM, M.F., BASIR, R., ASMAWI, M.Z., ROSIDAH AHMAD, M. and AKOWUAH, G.A. 2008. Antioxidant and hepatoprotective activities of Elephantopus tomentosus ethanol extract. Pharm. Biol. 46, 199– 206. YANG, D., WANG, Q., KE, L., JIANG, J. and YING, T. 2007. Antioxidant activities of various extracts of lotus (Nelumbo nuficera Gaertn) rhizome. Asia. Pac. J. Clin. Nutr. 16, 158– 163. Citing Literature Volume37, Issue2April 2013Pages 168-176 ReferencesRelatedInformation