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RNA polymerase associated with virions of pike fry rhabdovirus

1975; American Society for Microbiology; Volume: 15; Issue: 2 Linguagem: Inglês

10.1128/jvi.15.2.338-347.1975

1098-5514

Polly Roy, H. F. Clark, H P Madore, D. H. L. Bishop,

Viral Infections and Vectors

The association of an RNA-dependent RNA polymerase activity with virions of pike fry rhabdovirus has been demonstrated by both in vitro and in vivo studies. The temperature optimum for the in vitro assay is around 20 C, although enzyme activity can be observed at 4 C. Preparations of pike fry virus possess a glycoprotein, a membrane protein, a nucleoprotein, an L protein, and a phosphoprotein, as well as an RNA of about 3.8 times 10-6 mol wt. A protein kinase activity has been found associated with virus preparations. In vitro RNA product analyses indicate that the virus-associated enzyme functions principally as a transcriptase synthesizing viral-complementary, heteropolymeric RNA.