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The isolation of an RNA aptamer targeting to p53 protein with single amino acid mutation

2015; National Academy of Sciences; Volume: 112; Issue: 32 Linguagem: Inglês

10.1073/pnas.1502159112

1091-6490

Liang Chen, Farooq Rashid, Abdullah Shah, Hassaan Mehboob Awan, Mingming Wu, An Liu, Jun Wang, Tao Zhu, Zhaofeng Luo, Ge Shan,

MicroRNA in disease regulation

Significance We have developed a contrast screening strategy of systematic evolution of ligand exponential enrichment to isolate an RNA aptamer targeting specifically to p53R175H. This RNA aptamer partially rescued p53R175H in both cell cultures and tumor xenografts. p53 mutations are found in more than half of human cancers, and it is of biomedical significance to be able to isolate an RNA aptamer against a p53 mutation. This may also be the first report about an RNA aptamer specific to a mutant protein with a single amino acid change, and a plethora of human diseases are actually caused by single amino acid mutation. Thus, we present here a proof-of-concept study in isolating a specific and physiologically functional RNA aptamer against mutant protein with single amino acid substitution.