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Over-expression of ROR2 and Wnt5a cooperatively correlates with unfavorable prognosis in patients with non-small cell lung cancer

2015; Impact Journals LLC; Volume: 6; Issue: 28 Linguagem: Inglês

10.18632/oncotarget.4701

1949-2553

Chenlin Lu, Xudong Wang, Huijun Zhu, Jian Feng, Songshi Ni, Jianfei Huang,

Epigenetics and DNA Methylation

// Chenlin Lu 1, * , Xudong Wang 2, * , Huijun Zhu 3 , Jian Feng 1 , Songshi Ni 1 , Jianfei Huang 3 1 Department of Respiratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China 2 Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China 3 Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China * These authors have contributed equally to this work Correspondence to: Songshi Ni, e-mail: jsntnss@163.com Jianfei Huang, e-mail: ntyydoctor@126.com Keywords: RTK-like orphan receptor 2 (ROR2), Wnt5a, non-small cell lung cancer, immunohistochemistry, prognosis Received: March 08, 2015 Accepted: July 09, 2015 Published: July 21, 2015 ABSTRACT We investigated the expression of receptor tyrosine kinase-like orphan receptor (ROR) 2 and Wnt5a and their prognostic significance in non-small cell lung cancer (NSCLC). Tissue microarray-based immunohistochemical analysis was performed to determine the expression of ROR2 and Wnt5a in 219 patients. mRNA expression of ROR2 and Wnt5a was examined in 20 pairs of NSCLC and matched adjacent normal tissues by real-time PCR. Compared with non-tumorous tissues, both mRNA expression and protein product of ROR2 and Wnt5a genes were significantly increased in NSCLC. c 2 analysis revealed that high ROR2 or Wnt5a expression in NSCLC was significantly associated with advanced TNM stage. High expression of both ROR2 and Wnt5a was also related to advanced TNM stage. Multivariate analyses suggested that ROR2, Wnt5a and TNM stage were independent prognostic factors in NSCLC. Our clinical findings suggest that high ROR2 or Wnt5a expression is associated with poor prognosis in NSCLC, and combined detection of ROR2 and Wnt5a is helpful in predicting the prognosis of NSCLC.