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rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli.

1995; Springer Nature; Volume: 14; Issue: 5 Linguagem: Inglês

10.1002/j.1460-2075.1995.tb07084.x

1460-2075

Pierre E. Rouvière, Alejandro De Las Peñas, Joan Mecsas, C. Lu, Kenneth E. Rudd, Carol A. Gross,

RNA and protein synthesis mechanisms

Research Article1 March 1995free access rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli. P.E. Rouvière P.E. Rouvière Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author A. De Las Peñas A. De Las Peñas Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author J. Mecsas J. Mecsas Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author C.Z. Lu C.Z. Lu Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author K.E. Rudd K.E. Rudd Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author C.A. Gross C.A. Gross Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author P.E. Rouvière P.E. Rouvière Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author A. De Las Peñas A. De Las Peñas Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author J. Mecsas J. Mecsas Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author C.Z. Lu C.Z. Lu Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author K.E. Rudd K.E. Rudd Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author C.A. Gross C.A. Gross Department of Stomatology, University of California-San Francisco 94143-0512. Search for more papers by this author Author Information P.E. Rouvière1, A. De Las Peñas1, J. Mecsas1, C.Z. Lu1, K.E. Rudd1 and C.A. Gross1 1Department of Stomatology, University of California-San Francisco 94143-0512. The EMBO Journal (1995)14:1032-1042https://doi.org/10.1002/j.1460-2075.1995.tb07084.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: sigma 32 and sigma E. The sigma 32-regulated response is well understood, whereas little is known about that of sigma E, except that it responds to extracytoplasmic immature outer membrane proteins. To further understand this response, we located the rpoE gene at 55.5′ and analyzed the role of sigma E. sigma E is required at high temperature, and controls the transcription of at least 10 genes. Some of these might contribute to the integrity of the cell since delta rpoE cells are more sensitive to SDS plus EDTA and crystal violet. sigma E controls its own transcription from a sigma E-dependent promoter, indicating that rpoE transcription plays a role in the regulation of E sigma E activity. Indeed, under steady-state conditions, the transcription from this promoter mirrors the levels of E sigma E activity in the cell. However, it is unlikely that the rapid increase in E sigma E activity following induction can be accounted for solely by increased transcription of rpoE. Based upon homology arguments, we suggest that a gene encoding a negative regulator of sigma E activity is located immediately downstream of rpoE and may function as the target of the E sigma E inducing signal. Previous ArticleNext Article Volume 14Issue 51 March 1995In this issue RelatedDetailsLoading ...