Cytotoxic T cells in HIV2 seropositive Gambians. Identification of a virus-specific MHC-restricted peptide epitope.
1993; American Association of Immunologists; Volume: 151; Issue: 6 Linguagem: Inglês
10.4049/jimmunol.151.6.3361
ISSN1550-6606
AutoresF Gotch, Steve McAdam, C.E.M. Allsopp, Awen Gallimore, John Elvin, Marie Paule Kiény, AV Hill, Andrew J. McMichael, H. Whittle,
Tópico(s)vaccines and immunoinformatics approaches
ResumoA preliminary study of gag-specific, MHC-restricted CD8+ CTL has been performed in nine Gambian patients infected with HIV2. Such CTL were present in at least 55% of patients in fresh peripheral blood mononuclear cells without the requirement for in vitro restimulation. We have identified a nonamer peptide from HIV2 gag that is recognized by CD8+ HLA-B53 CTL using an amino acid sequence motif predicted from analysis of endogenous peptides eluted from HLA-B53 molecules. This peptide, from an HIV2/SIV conserved sequence, has previously been reported to be recognized by CTL from non-human primates vaccinated with recombinant vaccinia virus expressing the gag protein of SIV or infected with SIV virus. HLA-B53-restricted, HIV2 gag-specific CTL did not recognize target cells expressing HIV1 gag proteins, indicating that no cellular cross protection to HIV1 could be expected in this case.
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