Characterization of Five Microsatellites in the Siberian Sturgeon Acipenser Baerii from Aquaculture
2013; Agroprint Timisoara; Volume: 46; Issue: 1 Linguagem: Inglês
ISSN
2344-4576
AutoresSergiu Emil Georgescu, Oana Canareica, Gina Oana Popa, Andreea Dudu, Marieta Costache,
Tópico(s)Genetic and phenotypic traits in livestock
ResumoSturgeons, a class of fish with a particular scientific and commercial importance have been overexploited through overfishing and poaching, nowadays being at the brink of extinction. The developments of the molecular techniques allow the detection of intraspecific genetic differences and could ensure an increased ability to characterize the extent of genetic variation in sturgeon populations, both aquaculture and wildlife. In our study we optimized a protocol for microsatellites amplification in Siberian sturgeon, Acipenser baerii. The microsatellite markers were originally designed for the American lake sturgeon, Acipenser fulvescens, Adriatic sturgeon, Acipenser naccari and Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus. We successfully amplified five microsatellite loci (LS 19, LS 68, Aox 9, Aox 45, AnacE4) obtaining allele peaks of different sizes which were analyzed by capilary electrophoresis. Except the locus AnacE4 that presented a low polymorphism, the other four loci were displaying minimum 6 alleles for each locus. Therefore, caracterization of these new markers increase the panel of molecular tools available for fish farmers to monitoring levels of genetic variation within stocks.
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