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On the disruption of biochemical and biological assays by chemicals leaching from disposable laboratory plasticware

2012; NRC Research Press; Volume: 90; Issue: 6 Linguagem: Inglês

10.1139/y2012-049

1205-7541

Aldo Olivieri, Owen S. Degenhardt, G. Reid McDonald, Deepak Narang, Isabelle M. Paulsen, Janna L. Kozuska, Andrew Holt,

Effects and risks of endocrine disrupting chemicals

Plastic consumables, used universally in bioscience laboratories, are presumed inert with respect to bioassay outcomes. However, it is clear that many pipette tips, microfuge tubes, and other plastic disposables leach bioactive compounds into assay solutions, profoundly affecting data and experimental interpretation. In this paper we discuss the nature and sources of leachates and review several examples of compromised bioassay data that speak to the probable widespread nature of this largely unrecognised source of error. Strategies for minimizing leachate interferences are discussed.