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Genome-wide re-sequencing of multidrug-resistant Mycobacterium leprae Airaku-3

2014; Elsevier BV; Volume: 20; Issue: 10 Linguagem: Inglês

10.1111/1469-0691.12609

1469-0691

Pushpendra Singh, Andrej Benjak, Solenne Carat, M. Kai, Philippe Busso, Charlotte Avanzi, Alberto Paniz‐Mondolfi, Corinne Peter, Keith Harshman, Jacques Rougemont, Masao Matsuoka, Stewart T. Cole,

Tuberculosis Research and Epidemiology

Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug-resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South East Asian strains of M. leprae.