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Toxic effects of a modified montmorillonite clay on the human intestinal cell line Caco-2

2013; Wiley; Volume: 34; Issue: 6 Linguagem: Inglês

10.1002/jat.2945

1099-1263

Sara Maisanaba, Daniel Gutiérrez‐Praena, Silvia Pichardo, Francisco J. Moreno, María Jordá-Beneyto, Ana M. Cameán, Susana Aucejo, Ángeles Jos,

Nanoparticles: synthesis and applications

Journal of Applied ToxicologyVolume 34, Issue 6 p. 714-725 Research Article Toxic effects of a modified montmorillonite clay on the human intestinal cell line Caco-2 Sara Maisanaba, Sara Maisanaba Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorDaniel Gutiérrez-Praena, Daniel Gutiérrez-Praena Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorSilvia Pichardo, Corresponding Author Silvia Pichardo Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainCorrespondence to: Silvia Pichardo Sánchez, Area of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, Spain. E-mail: [email protected]Search for more papers by this authorF. Javier Moreno, F. Javier Moreno Department of Cellular Biology, Faculty of Biology, University of Seville, Avda. Reina Mercedes s/n, 41012 Seville, SpainSearch for more papers by this authorMaría Jordá, María Jordá Department of Packaging Materials and Systems, ITENE, C/ Albert Einstein 1, 46980 Paterna (Valencia), SpainSearch for more papers by this authorAna M. Cameán, Ana M. Cameán Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorSusana Aucejo, Susana Aucejo Department of Packaging Materials and Systems, ITENE, C/ Albert Einstein 1, 46980 Paterna (Valencia), SpainSearch for more papers by this authorÁngeles Jos, Ángeles Jos Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this author Sara Maisanaba, Sara Maisanaba Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorDaniel Gutiérrez-Praena, Daniel Gutiérrez-Praena Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorSilvia Pichardo, Corresponding Author Silvia Pichardo Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainCorrespondence to: Silvia Pichardo Sánchez, Area of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, Spain. E-mail: [email protected]Search for more papers by this authorF. Javier Moreno, F. Javier Moreno Department of Cellular Biology, Faculty of Biology, University of Seville, Avda. Reina Mercedes s/n, 41012 Seville, SpainSearch for more papers by this authorMaría Jordá, María Jordá Department of Packaging Materials and Systems, ITENE, C/ Albert Einstein 1, 46980 Paterna (Valencia), SpainSearch for more papers by this authorAna M. Cameán, Ana M. Cameán Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this authorSusana Aucejo, Susana Aucejo Department of Packaging Materials and Systems, ITENE, C/ Albert Einstein 1, 46980 Paterna (Valencia), SpainSearch for more papers by this authorÁngeles Jos, Ángeles Jos Department of Toxicology, Faculty of Pharmacy, University of Seville, Profesor García González n°2, 41012 Seville, SpainSearch for more papers by this author First published: 09 October 2013 https://doi.org/10.1002/jat.2945Citations: 57Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat ABSTRACT The incorporation of the natural mineral clay montmorillonite into polymeric systems enhances their barrier properties as well as their thermal and mechanical resistance, making them suitable for a wide range of industrial applications, e.g., in the food industry. Considering humans could easily be exposed to these clays due to migration into food, toxicological and health effects of clay exposure should be studied. In the present work, the cytotoxic effects induced by two different clays (the unmodified clay Cloisite® Na+, and the organically modified Cloisite® 30B) on Caco-2 cells were studied after 24 and 48 h of exposure. The basal cytotoxicity endpoints assessed were total protein content, neutral red uptake and a tetrazolium salt reduction. Our results showed that only Cloisite® 30B induced toxic effects. Therefore, the effects of subcytotoxic concentrations of this clay on the generation of intracellular reactive oxygen species, glutathione content and DNA damage (comet assay) were investigated. Results indicate that oxidative stress may be implicated in the toxicity induced by Closite® 30B, in regards of the increases in intracellular reactive oxygen species production and glutathione content at the highest concentration assayed, while no damage was observed in DNA. The most remarkable morphological alterations observed were dilated cisternae edge in the Golgi apparatus and nucleolar segregation, suggesting impairment in the secretory functions, which could be related to inhibition in the synthesis of proteins. Copyright © 2013 John Wiley & Sons, Ltd. References Afriyie-Gyawu E, Mackie J, Dash B, Wiles M, Taylor J, Huebner H, Tang L, Guan H, Wang J-S, Phillips TD. 2005. Chronic toxicological evaluation of dietary NovaSil Clay in Sprague–Dawley rats. Food Addit. Contam. 22: 259–269. 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