Portal de Periódicos da CAPES

Ability of Pythium oligandrum strains to protect Vitis vinifera L., by inducing plant resistance against Phaeomoniella chlamydospora, a pathogen involved in Esca, a grapevine trunk disease

2015; Elsevier BV; Volume: 92; Linguagem: Inglês

10.1016/j.biocontrol.2015.08.005

1090-2112

A. Yacoub, Jonathan Gerbore, Noël Magnin, Pascal Chambon, Marie‐Cécile Dufour, Marie‐France Corio‐Costet, Rémy Guyoneaud, Patrice Rey,

Plant-Microbe Interactions and Immunity

Biological control of Phaeomoniella chlamydospora, a pathogen involved in Esca, a grapevine trunk wood disease, was performed using the oomycete, Pythium oligandrum. Three 4-month greenhouse assays showed that necrosis of Vitis vinifera L. cv. Cabernet Sauvignon cuttings caused by P. chlamydospora was significantly reduced (40–50%) when P. oligandrum colonized the plant root systems. The expression of a set of 22 grapevine defense genes was then quantified by real-time polymerase chain reaction to determine plant responses in the interaction between P. oligandrum/V. vinifera L./P. chlamydospora. In the trunk, specific grapevine responses to the different treatments (control, P. oligandrum, P. chlamydospora and P. oligandrum + P. chlamydospora treatments) were significantly differentiated. Expression levels of 6 genes associated with P. chlamydospora infection showed higher induction than when plants were pre-treated with P. oligandrum. These genes are involved in various pathways (PR proteins, phenylpropanoid pathways, oxylipin and oxydo-reduction systems).