Purification and partial biochemical characterization of an edema inducing phospholipase A2 from Vipera russelli (Russell's viper) snake venom
1988; Elsevier BV; Volume: 26; Issue: 8 Linguagem: Inglês
10.1016/0041-0101(88)90278-4
ISSN1879-3150
AutoresBannikuppe S. Vishwanath, R. Manjunatha Kini, T. Veerabasappa Gowda,
Tópico(s)Ion channel regulation and function
ResumoPurification and partial biochemical characterization of an edema inducing phospholipase A2 from Vipera russelli (Russell's viper) snake venom. Toxicon26, 713 – 720, 1988. — A phospholipase A2 (VRV PL-VI) from Vipera russelli venom was purified to homogeneity in a single step on CM-Sephadex C-25 column. VRV PL-VI is a basic protein with a mol, wt of about 12,000 and showed a basic pH optimum and a high temperature maximum. It hydrolysed purified phospholipids in the order of phosphatidyl-ethanolamine > phosphatidylcholine > phosphatidylserine > phosphatidylinositol = 0. It is toxic with an LD50 value (i.p.) of 3.5 μg/g body weight in mice and it induced persistent edema in the mouse foot pad.
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