The Inhibition of Mitochondrial Peroxidation by Ubiquinone and Ubiquinol
1966; Elsevier BV; Volume: 241; Issue: 19 Linguagem: Inglês
10.1016/s0021-9258(18)99728-0
ISSN1083-351X
Autores Tópico(s)Free Radicals and Antioxidants
ResumoUbiquinol-6 (reduced coenzyme Q6) was more effective than ubiquinone-6 and almost as effective as α-tocopherol in reducing the stable free radical diphenyl-p-picrylhydrazyl. The antioxidant activity of ubiquinol-6 was measured at low oxygen concentrations with an oxygen electrode. Ubiquinol-6 was found to be as efficient as α-tocopherol as an inhibitor of the heme-catalyzed peroxidation of arachidonic acid emulsions. Ubiquinol-6 was oxidized to ubiquinone-6 by free radical products of the heme-catalyzed decomposition of cumene hydroperoxide. α-Tocopherol did not inhibit this reaction. Light was used to catalyze the peroxidation of mitochondrial lipids at low concentrations of oxygen. This peroxidation was inhibited by the addition of ubiquinol-6 or α-tocopherol to the mitochondria. Light-induced mitochondrial peroxidation was inhibited by ubiquinol-6 produced from exogenous ubiquinone-6 by the electron transport-linked oxidation of β-hydroxybutyrate. Ubiquinols may function as antioxidants in the mitochondrion. This could explain why ubiquinones and related compounds have been found effective in the relief of certain vitamin E deficiency syndromes in some species.
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