Portal de Periódicos da CAPES

Eubacterium limosum Activates Isoxanthohumol from Hops (Humulus lupulus L.) into the Potent Phytoestrogen 8-Prenylnaringenin In Vitro and in Rat Intestine3

2008; Elsevier BV; Volume: 138; Issue: 7 Linguagem: Inglês

10.1093/jn/138.7.1310

1541-6100

Sam Possemiers, Sylvie Rabot, Juan Carlos Espı́n, Aurélia Bruneau, Catherine Philippe, Antonio González‐Sarrías, Arne Heyerick, Francisco A. Tómas‐Barberán, Denis De Keukeleire, Willy Verstraete,

Phytoestrogen effects and research

Recently, it was shown that the exposure to the potent hop phytoestrogen 8-prenylnaringenin (8-PN) depends on intestinal bacterial activation of isoxanthohumol (IX), but this occurs in only one-third of tested individuals. As the butyrate-producing Eubacterium limosum can produce 8-PN from IX, a probiotic strategy was applied to investigate whether 8-PN production could be increased in low 8-PN producers, thus balancing phytoestrogen exposure. Using fecal samples from high (Hop +) and low (Hop −) 8-PN–producing individuals, a Hop + and Hop − dynamic intestinal model was developed. In parallel, Hop + and Hop − human microbiota-associated rats were developed, germ-free (GF) rats acting as negative controls. IX and then IX + E. limosum were administered in the intestinal model and to the rats, and changes in 8-PN production and exposure were assessed. After dosing IX, 80% was converted into 8-PN in the Hop + model and highest 8-PN production, plasma concentrations, and urinary and fecal excretion occurred in the Hop + rats. Administration of the bacterium triggered 8-PN production in the GF rats and increased 8-PN production in the Hop − model and Hop − rats. 8-PN excretion was similar in the feces (294.1 ± 132.2 nmol/d) and urine (8.5 ± 1.1 nmol/d ) of all rats (n = 18). In addition, butyrate production increased in all rats. In conclusion, intestinal microbiota determined 8-PN production and exposure after IX intake. Moreover, E. limosum administration increased 8-PN production in low producers, resulting in similar 8-PN production in all rats.