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ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors

2015; Nature Portfolio; Volume: 12; Issue: 10 Linguagem: Inglês

10.1038/nmeth.3542

1548-7105

Christian Schmidl, André F. Rendeiro, Nathan C. Sheffield, Christoph Bock,

Chromosomal and Genetic Variations

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.