Genetic Assay for Small Fragments of Bacteriophage φX174 Deoxyribonucleic Acid
1971; American Society for Microbiology; Volume: 8; Issue: 2 Linguagem: Inglês
10.1128/jvi.8.2.181-189.1971
ISSN1098-5514
AutoresClyde A. Hutchison, Marshall H. Edgell,
Tópico(s)Molecular Biology Techniques and Applications
ResumoThe double-stranded replicative form deoxyribonucleic acid (RF-DNA) of bacteriophage phiX174 was fragmented by pancreatic deoxyribonuclease, and the complementary strand fragments were then annealed to intact viral single strands. When such complexes infected Escherichia coli spheroplasts, some of the progeny virus bore genetic markers derived from the RF-DNA fragments. In this way, genetic markers have been salvaged from DNA fragments less than 50 nucleotides in length. This method is potentially useful as a specific assay to aid in the purification of genetically defined DNA fragments and also as a mechanism for the incorporation of small chemically synthesized DNA sequences into viral genomes.
Referência(s)