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Antimutagenic Activities of Cell Wall and Cytosol Fractions of Lactic Acid Bacteria Isolated from Kimchi

1998; Korean Society of Food Science and Nutrition; Volume: 3; Issue: 4 Linguagem: Inglês

2287-8602

Kun‐Young Park, So-Hee Kim, Tae-Jin Son,

Phytoestrogen effects and research

Cell wall(lactic acid bacteria-sonicated precipitate: LAB-SP) and cytosol (lactic acid bacteria-sonicated supernatant: LAB-SS) fractions were prepared from kimchi fermenting lactic acid bacteria such as Leuconostoc mesenteroides, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus plantarum and Pediococcus acidilactici, with Lactobacillus acidophillus isolated from yogurt. Using the Ames mutagenicity test and SOS chromotest system, the antimutagenic activity of those cell fractions was studied. One hundred eighty μl of LAB-SP from lactic acid bacteria isolated from kimchi, excepting Pediococcus acidilactici, supressed the mutagenicity of 4-nitroquinoline-1-oxide (4-NQO) in Ames mutagenicity test and SOS chromotest system, by above 90% and 60%, respectively. LAB-SP from lactic acid bacteria exhibited the antimutagenic activity aganist 2-amino-3,4-dimethyl-imidazo (4,5-f) quinoline (MeIQ) in Ames mutagenicity test, depending on the concentration. Especially, Lactobacillus plantarum which were isolated from kimchi had the strongest antimutagenicity on MeIQ. LAB-SP from lactic acid bacteria also inhibited the mutagenicity mediated by 3-amino-1-methyl-5H-pyrido [4,3-b]indole (Trp-P-2). Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus acidphillus had higher antimutagenicity against Trp-P-2 than the other lactic acid bacteria. However, LAB-SS of lactic acid bacteria did not show any mutagenic activity against 4-NQO in Ames mutagenicity test and SOS chromotest systems. On the mutagenicity of MeIQ and Trp-P-2, LAB-SS of lactic acid bacteria from kimchi or dairy products exhibited a weaker inhibitory effect than LAB-SP of those bacteria. These results represent that, whether the lactic acid bacteria from kimchi are viable or nonviable, antimutagenic activity was still effective. We suggest that the strong, antimutaganic activity of lactic acid bacteria might be found in the cell wall fraction, rather than in the cytosol fraction.