Portal de Periódicos da CAPES

Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast

2015; Cold Spring Harbor Laboratory Press; Volume: 2015; Issue: 6 Linguagem: Inglês

10.1101/pdb.prot085191

1940-3402

Jon-Matthew Belton, Job Dekker,

RNA and protein synthesis mechanisms

Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.