Artigo Acesso aberto Produção Nacional Revisado por pares

In vitro biomass production of Sideroxylon obtusifolium (Roem & Schult)

2008; Springer Science+Business Media; Volume: 18; Linguagem: Inglês

10.1590/s0102-695x2008000500010

ISSN

1981-528X

Autores

Annie Elizabeth Santiago Beltrão, Anna Cláudia de Andrade Tomaz, Fabiana Augusta Santiago Beltrão, Paulo Marinho,

Tópico(s)

Plant tissue culture and regeneration

Resumo

Sideroxylon obtusifolium belongs to the family Sapotaceae which occurs in the Southwest and Northeast of Brazil. It is used in folk medicine due to its anti-inflammatory and hypoglycemic properties. The intense use of its barks and the lack of cultivation of the plant made rare its occurrence in the natural habitat. This study was performed in order to get information about the biomass production of this native species using the techniques of plant tissues culture. The Murashige & Skoog (1962) and Gamborg et al. (1968) culture media containing auxin 2,4-dichlorophenoxyacetic and kinetin kin were used for callus induction. Fragments of leaves were used as explants and sterilized with sodium hypochlorite for 40 minutes. The best outcome for callus induction was obtained with concentrations of auxin/kinetin of 0.1 mg/L / 1 mg/L in the Murashige & Skoog culture medium.

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