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Generation of Epstein‐Barr Virus (EBV)–Immortalized B Cell Lines

2007; Wiley; Volume: 76; Issue: 1 Linguagem: Inglês

10.1002/0471142735.im0722s76

1934-368X

Giovanna Tosato, Jeffrey I. Cohen,

Parvovirus B19 Infection Studies

Abstract Immortalization of B lymphocytes by EBV is an effective procedure for inducing long‐term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.