Quantification of bovine IgG, IgM and IgA antibodies to B-toxin by enzyme immunoassay I. Preparturient immunization for enhancement of passive transfer of immunity
1983; Elsevier BV; Volume: 4; Issue: 5-6 Linguagem: Inglês
10.1016/0165-2427(83)90066-1
ISSN1873-2534
Autores Tópico(s)Microbial infections and disease research
ResumoA quantitative competitive binding “triple-sandwich” enzyme immunoassay was developed and used to evaluate pathogen/class-specific antibody responses in Holstein-Friesian cows vaccinated against Clostridium perfringens B-toxin. Vaccination of cows at six weeks and again at two weeks prepartum increased pathogen-specific IgG levels in each dam's colostrum and respective calf's serum. Pathogen-specific IgG and IgM concentrations in dams' sera and colostra were related to subsequent pathogen-specific IgG and IgM neonatal sera concentrations. Only pathogen-specific IgA in dams' colostra was correlated to neonatal levels, possibly owing to a different origin and role of this immunoglobulin class. All class-specific colostral immunoglobulin levels were related to subsequent neonatal concentrations. Isotypic antibody responses against C. perfringens B-toxin were found with pathogen-specific IgM predominant in dams' sera and pathogen-specific IgA predominant in colostra and neonatal sera.
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