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Teneurin‐4 is a novel suppressor of myogenesis (LB235)

2014; Wiley; Volume: 28; Issue: S1 Linguagem: Inglês

10.1096/fasebj.28.1_supplement.lb235

1530-6860

Kana Ishii, Nobuharu Suzuki, Yo Mabuchi, Chihiro Akazawa,

CAR-T cell therapy research

Teneurin‐4 is a novel suppressor of myogenesis K.Ishii, N.Suzuki, Y.Mabuchi, C.Akazawa : Depart. of Biochem. and Biophys., Grad. Sch. of Health Care Sci., TMDU, Tokyo, Japan Muscle satellite cells are maintained in the undifferentiated quiescent state, and initiate to proliferate and differentiate at the activated stage during muscle regeneration. It has been recently reported that the transmembrane protein teneurin‐4 (Ten‐4) is highly expressed in the quiescent satellite cells. However, its cellular and molecular function in myogenesis remains unknown. We therefore aimed to elucidate the function of Ten‐4 using the mouse myoblast cell line C2C12 and the primary culture of satellite cells. During differentiation of C2C12 cells, the peak of Ten‐4 expression was observed on day 2, while expression levels of myogenic differentiation markers, myogenin and myosin heavy chain (MHC), were elevated and peaked after day 5. Knocking down the Ten‐4 expression in C2C12 cells increased the expression levels of myogenin and MHC, and promoted the myotube formation 4 days after induction of differentiation. Further, Ki67‐positive proliferating cells were increased on day 2 in the Ten‐4‐knockdown C2C12 cells. Conversely, overexpression of Ten‐4 reduced the expression levels of myogenin and MHC, the formation of myotubes, and the number of Ki67‐positive cells. Together, our findings showed that Ten‐4 is a novel suppressor of proliferation and differentiation during myogenesis of C2C12 cells, which is consistent with the previous report regarding the Ten‐4 expression in quiescent satellite cells. These evidences suggest that Ten‐4 may be a crucial regulator of maintaining the stemness of muscle satellite cells.