Portal de Periódicos da CAPES

Selective Isolation of Mammalian Genes by TAR Cloning

2006; Wiley; Volume: 49; Issue: 1 Linguagem: Inglês

10.1002/0471142905.hg0517s49

1934-8266

Natalay Kouprina, Vladimir Larionov,

Animal Genetics and Reproduction

Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.