Reliable Measurement of Glycated Hemoglobin in Frozen Blood Samples: Implications for Epidemiologic Studies
2002; American Association for Clinical Chemistry; Volume: 48; Issue: 9 Linguagem: Inglês
10.1093/clinchem/48.9.1627
ISSN1530-8561
AutoresLinda Youngman, Sarah L. Clark, Susan E. Manley, Richard Peto, Rory Collins,
Tópico(s)Hyperglycemia and glycemic control in critically ill and hospitalized patients
ResumoAppropriately reliable estimates of the quantitative importance of various risk factors for chronic diseases and of the nature of any interactions between them may require epidemiologic studies involving several thousand “cases” of a disease. Blood-based epidemiologic studies on this scale can be facilitated by practicable methods for collection, storage, and analysis of very large numbers of samples. For example, although assessment of diabetic status using blood glucose concentration may require fasted samples, glycated hemoglobin (HbA1c) provides a measure of average glycemic control over the preceding months that can be determined reliably in nonfasted blood (1), which is more convenient for large-scale studies. HbA1c has, however, traditionally been measured in fresh whole blood, which has precluded its analysis in frozen samples stored in epidemiologic studies for future assays. In the present study, we investigated the measurement of HbA1c in frozen “buffy coat” samples (aliquots of white blood cells, taken from …
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