Characterization of Non-Type B Haemophilus influenzae Strains Isolated from Patients with Invasive Disease
2000; American Society for Microbiology; Volume: 38; Issue: 12 Linguagem: Inglês
10.1128/jcm.38.12.4649-4652.2000
ISSN1098-660X
AutoresMarina Cerquetti, Marta Luisa Ciofi degli Atti, Giovanna Renna, Alberto Eugenio Tozzi, M.L. Garlaschi, Paola Mastrantonio,
Tópico(s)Pneumonia and Respiratory Infections
ResumoForty-one non-type b Haemophilus influenzae isolates from cases of invasive disease were characterized. By PCR capsular genotyping, 33 nonencapsulated strains, 4 type f isolates, and 4 b(-) strains were identified. By pulsed-field gel electrophoresis, the nonencapsulated isolates exhibited great genetic heterogenicity, whereas the type f and the b(-) strains seemed to have a clonal spread. Occurrence of the hifA gene was found by PCR in 18% of the nonencapsulated, 50% of the b(-), and all of the type f strains. Hemagglutinating fimbriae were generally expressed by nonencapsulated isolates when fimbrial gene hifA was present. Two nonencapsulated isolates not susceptible to ampicillin were detected; no strains were positive for beta-lactamase production.
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