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Differential damage and repair responses of pubococcygeus and bulbospongiosus muscles in multiparous rabbits

2014; Wiley; Volume: 35; Issue: 2 Linguagem: Inglês

10.1002/nau.22702

1520-6777

Kenia López‐García, Estela Cuevas‐Romero, Octavio Sánchez‐García, Pablo Pacheco, Margarita Martı́nez–Gómez, Francisco Castelán,

Peripheral Nerve Disorders

Neurourology and UrodynamicsVolume 35, Issue 2 p. 180-185 Original Basic Science Article Differential damage and repair responses of pubococcygeus and bulbospongiosus muscles in multiparous rabbits Kenia López-García, Kenia López-García Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorEstela Cuevas, Estela Cuevas Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, MéxicoSearch for more papers by this authorOctavio Sánchez-García, Octavio Sánchez-García Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorPablo Pacheco, Pablo Pacheco Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México-DF, México Instituto de Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorMargarita Martínez-Gómez, Margarita Martínez-Gómez Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México-DF, MéxicoSearch for more papers by this authorFrancisco Castelán, Corresponding Author Francisco Castelán Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Correspondence to: Francisco Castelán, Ph.D. Centro Tlaxcala de Biología de la Conducta Universidad Autónoma de Tlaxcala, Carr. Fed. Tlaxcala-Puebla Km 1.5, s/n 90070-Tlaxcala México. E-mail: fcocastelan@uatx.mxSearch for more papers by this author Kenia López-García, Kenia López-García Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorEstela Cuevas, Estela Cuevas Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, MéxicoSearch for more papers by this authorOctavio Sánchez-García, Octavio Sánchez-García Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Doctorado en Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorPablo Pacheco, Pablo Pacheco Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México-DF, México Instituto de Neuroetología, Universidad Veracruzana, Xalapa, MéxicoSearch for more papers by this authorMargarita Martínez-Gómez, Margarita Martínez-Gómez Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México-DF, MéxicoSearch for more papers by this authorFrancisco Castelán, Corresponding Author Francisco Castelán Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Correspondence to: Francisco Castelán, Ph.D. Centro Tlaxcala de Biología de la Conducta Universidad Autónoma de Tlaxcala, Carr. Fed. Tlaxcala-Puebla Km 1.5, s/n 90070-Tlaxcala México. E-mail: fcocastelan@uatx.mxSearch for more papers by this author First published: 28 November 2014 https://doi.org/10.1002/nau.22702Citations: 8 Potential conflicts of interest: Nothing to disclose. Christopher Chapple led the peer-review process as the Associate Editor responsible for the paper. The present work was done at the Centro Tlaxcala de Biología de la Conducta from the Universidad Autónoma de Tlaxcala. Present address of Kenia López-García is Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México-DF, México. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Aim To determine the extent of damage and regeneration associated with multiparity on the pubococcygeus and bulbospongiosus muscles. Methods Age-matched virgin nulliparous and multiparous rabbits that were killed at days 3 and 20 after the fourth delivery were used to harvest pubococcygeus and bulbospongiosus muscles. The activity of the lysosomal enzyme β-glucuronidase was used as a muscle damage indicator. The number of immunoreactive myofiber-associated nuclei anti-Pax7, -MyoD, and -myogenin, as well as the anti-desmin immunoreactive area were measured in muscle sections to estimate some regenerative stages. Significant differences were considered at a P ≤ 0.05. Results The β-glucuronidase activity was increased at postpartum day 20 in the pubococcygeus muscle. This variable was unaltered in the bulbospongiosus muscles of multiparas regardless of the postpartum day on which this was measured. The number of immunoreactive nuclei anti-Pax7 in the pubococcygeus muscle was similar between nulliparas and multiparas, whilst those of anti-MyoD and anti-myogenin were increased at postpartum days 3 and 20. The same was true for these latter three markers evaluated in the bulbospongiosus muscles, supporting an ongoing regeneration. The desmin-positive percentage of muscle area per field was increased at postpartum day 20 in the pubococcygeus muscle, whilst such an increment was seen at postpartum days 3 and 20 in the bulbospongiosus muscles. Conclusions Damage and regeneration of the pubococcygeus and bulbospongiosus muscles are differently influenced by multiparity in rabbits. This could rely on the anatomical location, metabolism, myofiber composition, and muscle exertion during pregnancy and/or the delivery of each muscle. Neurourol. Urodynam. 35:180–185, 2016. © 2014 Wiley Periodicals, Inc. Citing Literature Volume35, Issue2February 2016Pages 180-185 RelatedInformation