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Physiological and biochemical characterization of Azospirillum brasilense strains commonly used as plant growth-promoting rhizobacteria

2014; Wiley; Volume: 54; Issue: 12 Linguagem: Inglês

10.1002/jobm.201400135

1521-4028

Luciana P. Di Salvo, Esdras Silva, K. R. S. Teixeira, Rosalba Esquivel Cote, María Alejandra Pereyra, Inés E. García de Salamone,

Nematode management and characterization studies

Journal of Basic MicrobiologyVolume 54, Issue 12 p. 1310-1321 Full Paper Physiological and biochemical characterization of Azospirillum brasilense strains commonly used as plant growth-promoting rhizobacteria Luciana P. Di Salvo, Luciana P. Di Salvo Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaSearch for more papers by this authorEsdras Silva, Esdras Silva Embrapa Agrobiologia, Seropédica, BrazilSearch for more papers by this authorKátia R.S. Teixeira, Kátia R.S. Teixeira Embrapa Agrobiologia, Seropédica, BrazilSearch for more papers by this authorRosalba Esquivel Cote, Rosalba Esquivel Cote Departamento de Biología, Laboratorio de Microbiología Experimental, México DF, MéxicoSearch for more papers by this authorM. Alejandra Pereyra, M. Alejandra Pereyra Laboratorio de Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, ArgentinaSearch for more papers by this authorInés E. García de Salamone, Corresponding Author Inés E. García de Salamone Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina Correspondence: Dr. Inés E. García de Salamone, Cátedra de Microbiología Agrícola, FAUBA, Avenida San Martín 4453, CABA, (C1417DSE), Argentina E-mail:igarcia@agro.uba.ar Phone/Fax: (54)11-4524-8061Search for more papers by this author Luciana P. Di Salvo, Luciana P. Di Salvo Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, ArgentinaSearch for more papers by this authorEsdras Silva, Esdras Silva Embrapa Agrobiologia, Seropédica, BrazilSearch for more papers by this authorKátia R.S. Teixeira, Kátia R.S. Teixeira Embrapa Agrobiologia, Seropédica, BrazilSearch for more papers by this authorRosalba Esquivel Cote, Rosalba Esquivel Cote Departamento de Biología, Laboratorio de Microbiología Experimental, México DF, MéxicoSearch for more papers by this authorM. Alejandra Pereyra, M. Alejandra Pereyra Laboratorio de Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, ArgentinaSearch for more papers by this authorInés E. García de Salamone, Corresponding Author Inés E. García de Salamone Cátedra de Microbiología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina Correspondence: Dr. Inés E. García de Salamone, Cátedra de Microbiología Agrícola, FAUBA, Avenida San Martín 4453, CABA, (C1417DSE), Argentina E-mail:igarcia@agro.uba.ar Phone/Fax: (54)11-4524-8061Search for more papers by this author First published: 19 August 2014 https://doi.org/10.1002/jobm.201400135Citations: 11Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Azospirillum is a plant growth-promoting rhizobacteria (PGPR) genus vastly studied and utilized as agriculture inoculants. Isolation of new strains under different environmental conditions allows the access to the genetic diversity and improves the success of inoculation procedures. Historically, the isolation of this genus has been performed by the use of some traditional culture media. In this work we characterized the physiology and biochemistry of five different A. brasilense strains, commonly used as cereal inoculants. The aim of this work is to contribute to pose into revision some concepts concerning the most used protocols to isolate and characterize this bacterium. We characterized their growth in different traditional and non-traditional culture media, evaluated some PGPR mechanisms and characterized their profiles of fatty acid methyl esters and carbon-source utilization. This work shows, for the first time, differences in both profiles, and ACC deaminase activity of A. brasilense strains. Also, we show unexpected results obtained in some of the evaluated culture media. Results obtained here and an exhaustive knowledge revision revealed that it is not appropriate to conclude about bacterial species without analyzing several strains. Also, it is necessary to continue developing studies and laboratory techniques to improve the isolation and characterization protocols. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description jobm201400135-sm-0001-SuppLeg.doc26.5 KB Figure Legends jobm201400135-sm-0002-SuppFig-S1.tif137.5 KB Figure S1. Evolutionary relationships of taxa based on 16S rDNA sequences of Azospirillum spp. jobm201400135-sm-0003-SuppFig-S2.doc4.6 MB Figure S2. Images of colonies and veil-like pellicles in some evaluated culture media. Round, wrinkled dry and scarlet red with a brighter halo colonies of Az39 strain in RC medium (A and A1). Typical colonies of A. brasilense strains in RC (B and B1), TYG (C1), and Nfb (D and D1) media. Non-typical colonies of Az39 strain in TYG (C) and Nfb (D2) media. Veil-like pellicles of A. brasilense strains are growing in Nfb ss (E), LGI ss (G), and JNfb ss (H) media. Black arrows point out the pellicles. No growth was observed in LGglu ss medium (F). Round, wrinkled dry, and white-colored colonies in LGglu medium (I and I2). Round, wrinkled dry, and pink-colored colonies of Cd strain in LGglu medium (I1). Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume54, Issue12December 2014Pages 1310-1321 RelatedInformation