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Both early and late Drosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene.

1988; Springer Nature; Volume: 7; Issue: 3 Linguagem: Inglês

10.1002/j.1460-2075.1988.tb02876.x

1460-2075

Charles P. Romano, Brigitta Bienz-Tadmor, Brian D. Mariani, Fotis C. Kafatos,

Hemiptera Insect Studies

Research Article1 March 1988free access Both early and late Drosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene. C. P. Romano C. P. Romano Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author B. Bienz-Tadmor B. Bienz-Tadmor Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author B. D. Mariani B. D. Mariani Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author F. C. Kafatos F. C. Kafatos Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author C. P. Romano C. P. Romano Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author B. Bienz-Tadmor B. Bienz-Tadmor Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author B. D. Mariani B. D. Mariani Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author F. C. Kafatos F. C. Kafatos Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author Author Information C. P. Romano1, B. Bienz-Tadmor1, B. D. Mariani1 and F. C. Kafatos1 1Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. The EMBO Journal (1988)7:783-790https://doi.org/10.1002/j.1460-2075.1988.tb02876.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info In vivo transformation studies have been performed using fusion constructs of chorion DNA and the alcohol dehydrogenase (Adh) structural gene. The results indicate that almost exclusively 5′ flanking DNA regions of the early (s36) and late (s15) chorion genes suffice for conferring normal chorion developmental specificity (sex, tissue and temporal) on the reporter gene. In the case of s15, the proximal 5′ flanking DNA up to position −370 is sufficient for specificity. However, quantitative analysis indicates that one or more elements within or downstream of the s15 gene are required, either transcriptionally or post-transcriptionally, for attainment of an mRNA level comparable to that of the endogenous s15 gene (corrected for amplification); in the absence of such element(s), the average level of Adh transcript produced by fusion gene constructs is 18-fold lower. Previous ArticleNext Article Volume 7Issue 31 March 1988In this issue RelatedDetailsLoading ...