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Deregulation of dicer and mir-155 expression in liposarcoma

2015; Impact Journals LLC; Volume: 6; Issue: 12 Linguagem: Inglês

10.18632/oncotarget.3201

1949-2553

Bruno Vincenzi, Michele Iuliani, Alice Zoccoli, Francesco Pantano, Marco Fioramonti, Delia De Lisi, Anna Maria Frezza, Carla Rabitti, Giuseppe Perrone, Andrea Onetti Muda, Antonio Russo, Antonio Giordano, Daniele Santini, Angelo Paolo Dei Tos, Giuseppe Tonini,

Circular RNAs in diseases

// Bruno Vincenzi 1 , Michele Iuliani 2 , Alice Zoccoli 2 , Francesco Pantano 1 , Marco Fioramonti 2 , Delia De Lisi 1 , Anna Maria Frezza 1 , Carla Rabitti 3 , Giuseppe Perrone 3 , Andrea Onetti Muda 3 , Antonio Russo 5 , Antonio Giordano 6, 7 , Daniele Santini 1 , Angelo Paolo Dei Tos 4 , Giuseppe Tonini 1 1 Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy 2 Translational Oncology Laboratory, Campus Bio-Medico University of Rome, Rome, Italy 3 Pathology, Campus Bio-Medico University of Rome, Rome, Italy 4 Anatomic Pathology, General Hospital of Treviso, Treviso, Italy 5 Department of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy 6 Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University BioLife Science, Philadelphia, PA, USA 7 Department of Medicine, Surgery & Neuroscience University of Siena, Italy Correspondence to: Antonio Russo, e-mail: antonio.russo@usa.net Keywords: Dicer, Drosha, mir-155, liposarcoma Received: December 19, 2014 Accepted: January 23, 2015 Published: April 04, 2015 ABSTRACT Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.