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Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory

1993; American Society for Microbiology; Volume: 31; Issue: 11 Linguagem: Inglês

10.1128/jcm.31.11.2882-2889.1993

1098-660X

Paul A. Kirschner, Burkhard Springer, Ulrich Vogel, A Meier, Annette Wrede, M Kiekenbeck, F C Bange, Erik C. Böttger,

Helicobacter pylori-related gastroenterology studies

Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.