Production of Egg Proteins, Enriched with L-Leucine-13C1, for the Study of Protein Assimilation in Humans Using the Breath Test Technique ,
1997; Elsevier BV; Volume: 127; Issue: 2 Linguagem: Inglês
10.1093/jn/127.2.327
ISSN1541-6100
AutoresPieter Evenepoel, Hiele Martin, Luypaerts Anja, Benny Geypens, B Johan, Decuypere Eddy, Rutgeerts Paul, Ghoos Yvo,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoProtein assimilation and metabolism studies are hindered by the lack of an adequate oral tracer, i.e., labeled proteins. We present a new and easily reproducible methodology for producing large amounts of egg proteins labeled with L-leucine-13C1. Laying hens were fed a 0.2% leucine-deficient food supplemented with 0.2% L-leucine-13C1 (99 atom %). At plateau, eggs containing highly enriched proteins were obtained. The 13C content of egg white relative to the total C content was 1.3371 atom %, corresponding to Δ = 206‰. The overall tracer recovery in egg proteins was high (40.2%), making this method financially attractive as well. Accurately measurable levels of 13CO2 in breath were obtained after ingestion of a physiological load of labeled egg white proteins. Thus, egg proteins with sufficient 13C enrichment and applicable for human protein assimilation and metabolism kinetic studies were produced in an easily reproducible and highly efficient manner.
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