Liquid chromatography of codeine in plasma with fluorescence detection.
1982; American Association for Clinical Chemistry; Volume: 28; Issue: 5 Linguagem: Inglês
10.1093/clinchem/28.5.1137
ISSN1530-8561
AutoresIrene Tsina, Marian Fass, J A Debban, Shaikh B. Matin,
Tópico(s)Nicotinic Acetylcholine Receptors Study
ResumoThis analytical method for easier determination of codeine in human plasma is based on "high-performance" liquid chromatography for separation and the natural fluorescence of codeine for detection. Codeine is extracted from alkalinized plasma with a mixture of hexane and dichloromethane, and the extract is further purified and chromatographed. The method can be used for routine assay of codeine at the concentrations of 10 micrograms/L or greater in human plasma. As little as 4 micrograms/L can be detected. Coefficients of variation for the assay of codeine in the concentration range of 10 to 100 micrograms/L were 2.2-7.4% (n = 6). We used this method to establish a concentration/time profile for plasma from a human volunteer after a 60-mg oral dose of codeine sulfate.
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