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Biochemical indices (RNA/DNA ratio and protein content) in studying the nutritional status of Ruditapes decussatus (Linnaeus 1758) juveniles

2010; Wiley; Volume: 42; Issue: 1 Linguagem: Inglês

10.1111/j.1365-2109.2010.02614.x

1365-2109

Salem Fathallah, Mohamed Néjib Medhioub, Amel Medhioub, Hamadi Boussetta,

Environmental Toxicology and Ecotoxicology

Aquaculture ResearchVolume 42, Issue 1 p. 139-146 Biochemical indices (RNA/DNA ratio and protein content) in studying the nutritional status of Ruditapes decussatus (Linnaeus 1758) juveniles Salem Fathallah, Salem Fathallah Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorMohamed Néjib Medhioub, Mohamed Néjib Medhioub Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorAmel Medhioub, Amel Medhioub Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorHamadi Boussetta, Hamadi Boussetta Ecole Superieur d'Horticulture de Chott Mariem, Laboratoire de Biochimie et d'Ecotoxicologie Environnementale, Chott-Meriem, Sousse, TunisieSearch for more papers by this author Salem Fathallah, Salem Fathallah Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorMohamed Néjib Medhioub, Mohamed Néjib Medhioub Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorAmel Medhioub, Amel Medhioub Institut National des sciences et Technologies de la Mer Centre de Monastir, Laboratoire d'aquaculture, Monastir, TunisieSearch for more papers by this authorHamadi Boussetta, Hamadi Boussetta Ecole Superieur d'Horticulture de Chott Mariem, Laboratoire de Biochimie et d'Ecotoxicologie Environnementale, Chott-Meriem, Sousse, TunisieSearch for more papers by this author First published: 08 October 2010 https://doi.org/10.1111/j.1365-2109.2010.02614.xCitations: 12 Correspondence: S Fathallah, Laboratoire d'Aquaculture – Institut, National des Sciences et Technologies de la Mer BP59, route de Khniss 5000, Monastir, Tunisie. E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract In this work, we investigated the nutritional status of Ruditapes decussatus juveniles under different rearing conditions, using biochemical indices (RNA/DNA ratio and protein content) and the linear instantaneous growth rate (IGR). Two experiments were conducted to study the effect of starvation, addition of substrate (sand) as rearing support and diet composition on somatic growth and biochemical indices. Results show that biochemical indices of fed juveniles were significantly (P<0.05) higher. Highest RNA/DNA values and protein content were recorded in fed juveniles reared with substrate (P<0.05); conversely, lowest values were recorded in starved ones reared without substrate. In the second experiment, the highest RNA/DNA ratio was recorded in juveniles fed with control algal mixture composed of species commonly used in bivalve nurseries [Isochrysis galbana (clone T-Iso), Chaetoceros calcitrans and Tetraselmis suecica] and lowest in those fed with algal species isolated from Tunisian coasts. This confirms the usefulness of the RNA/DNA ratio and protein content in the clam's nutritional status evaluation. This assay is useful to provide information about the nutritional status and health of the clam in field conditions. References Albentosa M., Pèrez-Camacho A., Labarta U. & Fernandez-Reiriz M.J. 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