Expression of human pancreatic secretory trypsin inhibitor in Saccharomyces cerevisiae
1987; Elsevier BV; Volume: 59; Issue: 2-3 Linguagem: Inglês
10.1016/0378-1119(87)90323-4
ISSN1879-0038
AutoresIzumoto Yoshitaka, Takaaki Sato, Yaniamoto Tatsuo, Nobuo Yoshida, Kikuchi Norihisa, Michio Ogawa, Kenichi Matsubara,
Tópico(s)Peptidase Inhibition and Analysis
ResumoHuman pancreatic secretory trypsin inhibitor (PSTI) cDNA was expressed in Saccharomyces cerevisiae using the yeast acid phosphatase PH05 promoter. The product encoded by the PSTI-coding cDNA was correctly processed m yeast cells, and the PSTI molecules were efficiently secreted into the medium. The amino acid composition and the N-terminal amino acid sequence of the secreted PSTI molecules were identical to those of the authentic PSTI polypeptides from human pancreas, and the product exhibited trypsin-inhibitory activity.
Referência(s)