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DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease

1998; Elsevier BV; Volume: 10; Issue: 3 Linguagem: Inglês

10.1016/s0927-7765(97)00058-1

1873-4367

Mamoru Hatakeyama, Kaai Nakamura, S. Iwato, Hiroshi Handa, Keiji Fujimoto, Haruma Kawaguchi,

Genetically Modified Organisms Research

In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.