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Simultaneous determination of loratadine and pseudoephedrine sulfate in human plasma by liquid chromatography–electrospray mass spectrometry for pharmacokinetic studies

2005; Elsevier BV; Volume: 39; Issue: 1-2 Linguagem: Inglês

10.1016/j.jpba.2005.03.041

1873-264X

Jianguo Sun, Guangji Wang, Wei Wang, Shuai Zhao, Yi Gu, Jinwen Zhang, Mingwen Huang, Shao Fen, Hao Li, Qi Zhang, Haitang Xie,

Pesticide Residue Analysis and Safety

To support the pharmacokinetic and bioavailability study of an extended-release loratadine (LOR)/pseudoephedrine sulfate (PES) tablet, a high performance liquid chromatographic-electrospray ionisation–mass spectrometric method (LC–MS) was developed for the simultaneous determination of LOR and PES in human plasma. Diazepam (DP) and phenylpropanolamine (PPA) were used as internal standards for LOR and PES, respectively. Analytes were extracted from alkalized human plasma by liquid/liquid extraction using ethyl ether. Chromatographic separation was performed on an ODS column at flow rate of 0.2 ml/min. The total chromatographic run time was 10.5 min with the retention time of 7.1 min and 6.2 min for LOR and DP, respectively, and 2.2 min for both of PES and PPA. The LOQ was 10 pg/ml and 50 pg/ml for LOR and PES, respectively. The method is accurate and precise enough for its intended purpose.