Synthetic RNA-dependent DNA polymerase from calf thymus
1974; Elsevier BV; Volume: 60; Issue: 4 Linguagem: Inglês
10.1016/0006-291x(74)90325-8
ISSN1090-2104
AutoresShonen Yoshida, Teruo Ando, Toyoko Kondo,
Tópico(s)Genetics, Bioinformatics, and Biomedical Research
ResumoA RNA dependent-DNA polymerase was purified about 450-fold from the soluble fraction of calf thymus. This enzyme was able to copy the polyribonucleic acid strand of synthetic ribonucleic acid primed with complementary oligodeoxynucleotides, i.e., poly(rA)·(dT)10. This enzyme activity was separated from the DNA-dependent DNA polymerases by both DEAE-cellulose columm chromatography and glycerol gradient centrifugation. Some properties of this enzyme were described.
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