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Photometric assay of hepatic epoxide hydrolase activity with safrole oxide (SAFO) as substrate

1974; Elsevier BV; Volume: 23; Issue: 20 Linguagem: Inglês

10.1016/0006-2952(74)90058-6

1873-2968

Tadashi Watabe, Kazuko Akamatsu,

Hemoglobin structure and function

A simple and sensitive method for the assay of hepatic microsomal epoxide hydrolase activity by using safrole oxide (SAFO) as the substrate is described. It involves differential extraction of the unreacted substrate with n-hexane from the incubation medium containing the reaction product, safrole glycol (SAFG) followed by measurement of absorbancy at 288 nm of the separated hexane layer. A stoichiometric relationship between the SAFO disappearance and SAFG formation was confirmed in the enzymatic reaction.