Formation of androstanediol from 13C-labeled testosterone in humans
1990; Elsevier BV; Volume: 55; Issue: 4 Linguagem: Inglês
10.1016/0039-128x(90)90107-m
ISSN1878-5867
Autores Tópico(s)Hormonal Regulation and Hypertension
ResumoThe determination of urinary 5α-androstane-3α,17β-diol (3a-Diol) by gas chromatography/mass spectrometry during and after the infusion of stable-labeled testosterone (T) represents an alternative to the use of radioactive label for turnover studies in vivo. Using this methodology to assess the urinary excretion rates of T and 3a-Diol in healthy men (n = 6) and women (n = 5) during and after the intravenous infusion (t = 4 hours) of 20 mg (men) or 5 mg (women) [13C]testosterone, the cumulative renal excretion of 13C-labeled T was found to be 15.6 ± 9.6 μg/24 hours (men) and 1.1 ± 1.6 μg/24 hours (women), equivalent to 0.08% ± 0.05% 4 and 0.02% ± 0.03% of the infused amount of 13C-T, respectively. The cumulative excretion of 13C-3a-Diol was 67.7 ± 19.9 μg/24 hours (men) and 10.0 ± 6.0 μg/24 hours (women), equivalent to 0.3 ± 0.1% and 0.2 ± 0.1% of the infused dose of 13C-labeled testosterone, respectively.
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