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New isolation method of retina and interphotoreceptor matrix

1989; Elsevier BV; Volume: 49; Issue: 2 Linguagem: Inglês

10.1016/0014-4835(89)90101-2

1096-0007

F Uehara, Douglas Yasumura, Matthew M. LaVail,

Retinal Diseases and Treatments

The effect of high n − 3 (5.8%, w/w) vs. a low n − 3 (1.2%, w/w) fatty acids in a diet with a low ratio of polyunsaturated/saturated fatty acids (P/S = 0.27) content was investigated to determine the effect of diet on the level of long-and very-long-chain fatty acids (VLCFAC ⩾ 24) in phospholipids of rod outer segments (ROS) of normal and diabetic rats. After 6 weeks of feeding, diets high in n − 3 fatty acids increased the levels of 22:5(n − 3) and 22:6(n − 3), while decreasing the 22:5(n − 6) level in all major phospholipid classes, n − 6 and n − 3 VLCFA of C24 to C34 with 4, 5 and 6 double bonds were found only in phosphatidylcholine (PC) while other phospholipid classes contained only C24 fatty acids as minor components. The content of VLCFA in PC was approx. 6.7% (w/w) of total fatty acids in the ROS. Feeding a high n − 3 fatty acid diet significantly reduced n − 6 tetraenoic VLCFA in all phospholipids. In the diabetic state, the levels of n − 6 tetraenes and pentaenes in individual phospholipids were different from control animals. This study demonstrates that the VLCFA content of photoreceptor cells reflects the dietary level of n − 3 fatty acids fed. The unique polyenoic n − 6 and n − 3 VLCFA appear to be synthesized from shorter chain precursors which respond to altering the ratio of soln − 6n − 3 fatty acids fed.